First Report of <i>Fusarium proliferatum</i> Causing Garlic Clove Rot in Russian Federation

HomePlant DiseaseAhead of PrintFirst Report Fusarium proliferatum Causing Garlic Clove Rot in Russian Federation PreviousNext DISEASE NOTE OPENOpen Access licenseFirst FederationOlga K. Anisimova, Timofey M. Seredin, Olga A. Danilova, and Mikhail FilyushinOlga AnisimovaFederal Research Centre “Fundamentals Biotechnology” the Academy Sciences, Moscow 119071, RussiaSearch for more papers by this author, SeredinFederal Scientific Vegetable Center, Vniissok 143080, DanilovaFederal Filyushin†Corresponding author: Filyushin; E-mail Address: [email protected]://orcid.org/0000-0003-3668-7601Federal author AffiliationsAuthors Affiliations Anisimova1 Seredin2 Danilova1 Filyushin1 † 1Federal Russia 2Federal Published Online:26 Oct 2021https://doi.org/10.1094/PDIS-12-20-2743-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleGarlic (Allium sativum L.) is a widely consumed bulbous crop both worldwide Russia. About 200,000 tons garlic produced annually (https://rosstat.gov.ru/). Significant pre- postharvest losses regularly occur due sp. (Taylor et al. 2013). Since September 2018, rotting has been observed during bulb storage (data Federal FSVC, Region). The outer surface looked healthy, but underneath integumentary scales, cloves had light brown spots. When grown, diseased plants were characterized root disruption leaf drying; some cultivars, up 100% died. In January 2020, cultivars Strelets Dubkovsky bulbs, collected July 2019, with rot symptoms, taken from FSVC storage. Necrotic clove tissue fragments (0.2 0.5 cm) cut, sanitized 70% ethanol 3 min, rinsed sterile water, incubated on potato dextrose agar (PDA) 1 mg/ml ampicillin at 22°C dark. Four single-spore cultures obtained four bulbs. After 6 days incubation, isolates abundant aerial white mycelia acquired purple pigmentation. hyphae hyaline septation. All (Dubkovsky, 2, Strelets, 2) numerous oval unicellular microconidia without septa, 4.1 11.6 × 1.3 3.4 µm (n = 50) very few macroconidia three septa (21 26 4 [n 30]), narrowed ends. cultural conidial characteristics corresponded species (Leslie Summerell 2006). To determine species, DNA was extracted isolates, internal transcribed spacer (ITS), genes translation elongation factor 1α (EF1α) subunits 2 DNA-directed RNA polymerase II (RPB1 RPB2) amplified sequenced primers ITS1/ITS4 (White 1990), EF1/EF2 (O’Donnell 1998a), RPB1-F5/RPB1-R8 2010), fRPB2-5F/fRPB2-7cR (Liu 1999). sequences identical all isolates. isolate deposited NCBI GenBank (MW149129 [ITS], MW161161 [EF1α], MW413302 [RPB1], MW413303 [RPB2]); their analysis MLST (https://fusarium.mycobank.org/) showed 98.8 99.8% similarity (NRRL 13582, 13598, others), which part F. fujikuroi complex 1998b). test pathogenicity performed two times according method Leyronas (2018). For this, replicates 10 (cv. Strelets) soaked suspension (∼106 conidia/ml; isolate) 24 h. Ten control water. Petri dishes (five dish; filter paper wetted water) dark 23°C. 5 days, lesions mycelium developed treated cloves. taxonomic status fungus isolated necrotic determined as ITS, EF1α, RPB1, RPB2. basal known be caused oxysporum f. cepae (Snowdon 1990). This study first report causing bulbs produces variety mycotoxins infestation, our findings are important diagnosing disease because use food medicine.The author(s) declare no conflict interest.References:Leslie, J. F., Summerell, B. 2006. Page 224 in: Laboratory Manual. Blackwell, Oxford, U.K. https://doi.org/10.1002/9780470278376 Crossref, Google ScholarLeyronas, C., 2018. Plant Dis. 102:2658. https://doi.org/10.1094/PDIS-06-18-0962-PDN Link, ISI, ScholarLiu, Y. J., 1999. Mol. Biol. Evol. 16:1799. https://doi.org/10.1093/oxfordjournals.molbev.a026092 ScholarO’Donnell, K., 1998a. Proc. Natl. Acad. Sci. U.S.A. 95:2044. https://doi.org/10.1073/pnas.95.5.2044 1998b. Mycologia 90:465. 2010. Clin. Microbiol. 48:3708. https://doi.org/10.1128/JCM.00989-10 ScholarSnowdon, L. 1990. 250 A Color Atlas Post-Harvest Diseases Disorders Fruits Vegetables, Vol. 1. Wolfe Scientific, London, ScholarTaylor, A., 2013. Pathol. 62:103. https://doi.org/10.1111/j.1365-3059.2012.02624.x ScholarWhite, T. 315 PCR Protocols: Guide Methods Applications. Academic Press, San Diego, CA. ScholarThe interest.Funding: reported funded Foundation Basic Research, project number 20-316-70009.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Downloaded 602 Article History Published: 2021First Look: 9 Apr 2021Accepted: 2021 Information© American Phytopathological SocietyFundingRussian ResearchGrant/Award Number: 20-316-70009KeywordsFusarium proliferatumfungivegetablespathogen detectiongarlicThe interest.Cited ByPathogenesis-Related Genes PR1, PR2, PR4, PR5 Families Are Involved Response Infection L.)22 June | International Journal Molecular 22, No. 13Identification pathogenic fungi zone plant growth28 crops Russia, 3Incidence Etiology Postharvest Fungal Associated Bulb (Alllium sativum) Spain12 May Foods, 10,